PA113X Nerve Growth Factor beta (NGF beta) antigen
see all 12 Nerve Growth Factor beta products
20 µg / US$ 355
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Human Nerve Growth Factor beta (NGF beta)
Product Description
Human Nerve Growth Factor beta (NGF beta)
Properties
| Species | Human |
| Purity | >98% |
| Source | E. coli |
| Catalog Number | PA113X |
| Quantity | 20 µg |
| Price | US$ 355 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  PA113X.pdf |
Datasheet Extract
| Alternate names | Nerve Growth Factor beta (NGF beta)
|
| Background | Beta-NGF is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Beta-NGF is a potent neurotrophic factor that signals through its receptor beta-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. Beta-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival. |
| Source | E.coli |
| Format | State: Lyophilized purified fraction (>98% pure by SDS-PAGE and HPLC analyses) wit no preservatives. Endotoxinlevel: < 0.1 ng per µg (1EU/µg) Reconstitution: Restore in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 2-8°C for 1 week or -20°C for future use. |
| Description | The functional form of human beta-NGF is a noncovalently disulfide-linked homodimer, of two 13.5 kDa polypeptide monomers (238 total amino acid residues). The three disulfide bonds are required for biological activity. AASequence: SSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVR Biological Activity: determined by its ability to stimulate chick E9 DRG neurite outgrowth. The ED50 is < 1.0 ng/ml. Specific Activity: > 1 x 10e6 units/mg |
| Add. Information | Centrifuge the vial prior to opening! |
| Storage | Store the lyophilized protein at -20°C.
Reconstituted human beta-NGF should be stored in working aliquots with a carrier protein at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Jiang, Y. Nerve growth factor promotes TLR4 signaling-induced maturation of human dendritic cells in vitro through inducible p75NTR 1. The Journal of Immunology.;179(9):6297-304. 11/1/2007 17947706
2. Denham M, A murine respiratory-inducing niche displays variable efficiency across human and mouse embryonic stem cell species Am J Physiol Lung Cell Mol Physiol. ;292(5):L1241-7 5/1/2007 17220377 3. Fogarty, M.P. Fibroblast growth factor blocks Sonic hedgehog signaling in neuronal precursors and tumor cells. Proceedings of the National Academy of Sciences of the U S A.;104(8):2973-8. 2/20/2007 17299056 4. Zhang, J. Cyclic-AMP response element-based signaling assays for characterization of Trk family tyrosine kinases modulators. Neurosignals. 2006-2007;15(1):26-39. 7/1/2006 16837782 5. Leon A QN1/KIAA1009: a new essential protein for chromosome segregation and mitotic spindle assembly Oncogene. ;25(13):1887-95 3/23/2006 16302001 6. Pyle AD Neurotrophins mediate human embryonic stem cell survival Nat Biotechnol. ;24(3):344-50 3/1/2006 16444268 7. Piu F Beta-arrestin 2 modulates the activity of nuclear receptor RAR beta2 through activation of ERK2 kinase Oncogene. ;25(2):218-29 1/12/2006 16170358 8. Marco Rusnati Selective recognition of fibroblast growth factor-2 by the long pentraxin PTX3 inhibits angiogenesis Blood, Vol: 104 (1) pp. 92-99 7/1/2004 15031207 9. Claudio Rivera BDNF-induced TrkB activation down-regulates the K+-Cl- cotransporter KCC2 and impairs neuronal Cl- extrusion J. of Cell Bio. Vol:159 (5) pp. 747-752 12/9/2002 12473684 10. Fernandez PA Evidence that Axon-Derived Neuregulin Promotes Oligodendrocyte Survival in the Developing Rat Optic Nerve Neuron. Volume 28. Pp.81-90 10/1/2000 11086985 11. Coulsen EJ Chopper, a New Death Domain of the p75 Neurotrophin Receptor That Mediates Rapid Neuronal Cell Death*- J. Biol. Chem. Volume 275. Issue 39. Pp.30537-30545 9/29/2000 10882742 12. Yoshimura, S. Inhibition of neutral sphingomyelinase activation and ceramide formation by glutathione in hypoxic PC12 cell death. Journal of Neurochemistry.;73(2):675-83 8/1/1999 10428064 13. Rossi J Retarded Growth and Deficits in the Enteric and Parasympathetic Nervous System in Mice Lacking GFRa2, a Functional Neurturin Receptor Neuron, Volume 22. Pp.243-252 2/1/1999 10069331 14. Goff JP Influence of Cytokines on the Growth Kinetics and Immunophenotype of Daughter Cells resulting From the First Division of Single CD34+Thy-1+lin-Cells Blood, Vol 92. Number 11. Pp. 4098-4107 12/1/1998 9834215 15. Meyer-Franke A Depolarization and cAMP Elevation Rapidly Recruit TrkB to the Plasma Membrane of CNS Neurons Neuron, Volume 21-pp.681-693 10/1/1998 9808456 16. Martin G. Hanson Cyclic AMP Elevation Is Sufficient to Promote the Survival of Spinal Motor Neurons In Vitro The J. of Neuroscience Vol: 18(18): pp.7361-7371 9/15/1998 9736656 17. Cheng L Cultured Schwann Cells Constitutively Express the Myelin Protein Po Neuron. Volume 16. Pp. 309-319 By Cell Press 2/16/1996 8789946. |
