PA1352X Lithostathine 1 alpha (REG1A) antigen
see all 5 Lithostathine 1 alpha products
10 µg / US$ 305
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Human Lithostathine 1 alpha (REG1A)
Product Description
Human Lithostathine 1 alpha (REG1A)
Properties
| Presentation | Purified |
| Species | Human |
| Purity | >90% |
| Source | E. coli |
| Molecular Weight | 54 kD |
| Catalog Number | PA1352X |
| Quantity | 10 µg |
| Price | US$ 305 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  PA1352X.pdf |
Datasheet Extract
| Background | REG protein was shown to be stimulated during the regeneration of pancreatic islets. Since then, many Reg-related proteins have been identified in humans and other animals. In human, the four REG family genes, i.e., REG 1 alpha, REG 1 beta, REG-related sequence (RS) and HIP/PAP, have so far been isolated. These Reg-related proteins are classified into four subfamilies according to their amino-acid sequences, but they share a similar structure and physiological function. Reg protein is a growth factor for pancreatic beta cells and also suggest that the administration of Reg protein could be used as another therapeutic approach for diabetes mellitus. human REG cDNA which encoded a 166-amino acid protein with a 22-amino acid signal peptide. The amino acid sequence of human REG protein has 68% homology to that of rat Reg protein.
Reg I was found to be expressed mainly in pancreatic beta and acinoductular cells as well as gastric fundic enterochromaffin-like (ECL) cells. Reg I production in ECL cells is stimulated by gastrin, as well as by the proinflammatory cytokine, cytokine-induced neutrophil chemoattractant (CINC)-2Beta. In patients with chronic hypergastrinemia, Reg production is stimulated, with the increased proliferation of gastric mucosal cells. Patients with Helicobacter pylori infection also showed increased Reg production in the gastric mucosa, partly via increased plasma gastrin concentration and partly via increased proinflammatory cytokine production. The serum concentration of the reg-protein was significantly higher in patients with various pancreatic diseases than in normal controls, and was also significantly higher in patients with acute pancreatitis or chronic relapsing pancreatitis than in patients with chronic pancreatitis. Furthermore, the serum PSP/reg-protein concentration was also significantly increased in liver cirrhosis, choledocholithiasis, and various cancers of the digestive system. |
| Source | E. coli |
| Format | State: Sterile filtered and lyophilized from 0.5 mg/ml in 5mM Tris, 25mM NaCl, pH 7.5 Purity: > 95% (SDS-PAGE analyzed). Purification Method: Two-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography. Reconstitution: Add 0.2 ml of deionized H2O and let the lyophilized pellet dissolve completely |
| Applications | Western blotting.
ELISA. |
| Description | The Recombinant Human REG 1 alpha is produced with N-terminal fusion HisTag.
The Recombinant Human REG 1 alpha His-Tagged Fusion Protein has a molecular weight of 17.8 kDa protein containing 144 amino acid residues of the Human REG 1 alpha and 12 additional amino acid residues - HisTag (underlined). AASequence: MKHHHHHHAS HMQEAQTELP QARISCPEGT NAYRSYCYYF NEDRETWVDA DLYCQNMNSG NLVSVLTQAE GAFVASLIKE SGTDDFNVWI GLHDPKKNRR WHWSSGSLVS YKSWGIGAPS SVNPGYCVSL TSSTGFQKWK DVPCEDKFSF VCKFKN The amino acid sequence of the recombinant Human REG 1 alpha is 100% homologous to the amino acid sequence of the Human REG 1 alpha without signal sequence. |
| Storage | Lyophilized protein is stable at -20°C for one year from despatch.
After reconstitution the protein can be stored undiluted at 2-8°C for two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
| References | 1. Kinoshita Y, Ishihara S, Kadowaki Y, Fukui H, Chiba T. Reg protein is a unique growth factor of gastric mucosal cells. J Gastroenterol. 2004 Jun;39(6):507-13.
2. Zhang YW, Ding LS, Lai MD. Reg gene family and human diseases. World J Gastroenterol. 2003 Dec;9(12):2635-41. 3. Okamoto H. The Reg gene family and Reg proteins: with special attention to the regeneration of pancreatic beta-cells. J Hepatobiliary Pancreat Surg. 1999;6(3):254-62. 4. Boonyasrisawat W, Pulsawat P, Yenchitsomanus PT, Vannasaeng S, Pramukkul P,Deerochanawong C, Sriussadaporn S, Ploybutr S, Pasurakul T, Banchuin N :Analysis of the reg1alpha and reg1beta gene transcripts in patients with fibrocalculous ancreatopathy. Southeast Asian J Trop Med Public Health. 2002 Jun;33(2):365-72. |
