PA1376 Ubiquitin antigen
see all 332 Ubiquitin products
20 µg / US$ 225
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Human Ubiquitin
Product Description
Human Ubiquitin
Properties
| Presentation | Purified |
| Species | Human |
| Purity | >90% |
| Source | E. coli |
| Molecular Weight | 35 kD |
| Catalog Number | PA1376 |
| Quantity | 20 µg |
| Price | US$ 225 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  PA1376.pdf |
Datasheet Extract
| Concentration | 1.0 mg/ml |
| Background | Recombinant Human Ubiquitin (Ub) is a small protein composed of 76 amino acids. Ub is found only in eukaryotic organisms among which it shows a strong sequence conservation. The protein is present in all cell types, thus giving rise to its name. Ub is found either in free form or conjugated to proteins through a covalent bond between the glycine at the C-terminal end of Ub and the side chains of lysine on the proteins. The attachment of multiple copies of ubiquitin targets the proteins for degradation by the 26S proteosome. Ubiquitin ligation is an ATP-dependent multi-step process: Ub is activated by the E1 enzyme, the attachment of Ub to the target protein is catalyzed by the E2 enzyme acting in concert with E3 which plays a role in recognizing the substrate protein. |
| Source | Escherichia coli |
| Format | State: Liquid purified protein Purity: minimum 95% as estimated by analysis of Coomassie blue stained SDS-PAGE BufferSystem: 50mM HEPES (pH7.5) 150mM NaCl, 1mM DTT and 10%glycerol |
| Description | Recombinant human ubiquitin expressed in Escherichia coli and purified by ion-exchange chromatography. AASequence: MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG Molecular weight: 8.6kDa |
| Storage | Store the protein undiluted at 2-8°C for one week or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Haas AL, Siepmann TJ (1997) Pathways of ubiquitin conjugation. FASEB J. 11(14):1257-68.
2. Hershko A, Ciechanover A (1998) The ubiquitin system. Annu Rev Biochem. 67:425-79. 3. Pickart CM (2000) Ubiquitin in chains. Trends Biochem Sci. 25(11):544-8. 4. Pickart CM, Eddins MJ (2004) Ubiquitin: structures, functions, mechanisms. Biochim Biophys Acta. 1695(1-3):55-72. 5. Roos-Mattjus P, Sistonen L (2004) The ubiquitin-proteasome pathway. Ann Med. 36(4):285-95. |
