PA503X Agouti Related Protein (AGRP) (His-tagged Fusion Protein) antigen
see all 12 Agouti Related Protein products
0.1 mg / US$ 565
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Human Agouti Related Protein (AGRP) (His-tagged Fusion Protein)
Synonyms
AGRT, ART,
Product Description
Human Agouti Related Protein (AGRP) (His-tagged Fusion Protein)
Properties
| Presentation | Purified |
| Species | Human |
| Purity | >95% |
| Source | E. coli |
| Catalog Number | PA503X |
| Swiss Prot. No. | O00253 |
| Quantity | 0.1 mg |
| Price | US$ 565 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  PA503X.pdf |
Datasheet Extract
| Alternate names | AGRP |
| Concentration | 0.5 mg/mL |
| Background | Agouti-related protein is an endogenous antagonist of hypothalamic alpha-melanocortin receptors MC3R and MC4R with potent orexigenic activity. Although a complete deletion of the AGRP gene does not produce any significant metabolic phenotypes, reduction in AGRP expression by RNA interference is associated with increased metabolic rate along with reduced weight gain. In hypothalamus, it is produced by neurons in the medial portion of arcuate nucleus, which produce also the potent orexigenic peptide Neuropeptide Y (NP-Y). Another site of central AGRP production is the hypothalamic nucleus. AGRP encompasses 132 amino acid residues and its alpha-melanocortin inhibiting activity results in a 34 amino acid cystine knot domain within the C-terminal (87-132) portion of the protein. Both AGRP and NP-Y expression was shown to be supressed by leptin. Central administration of AGRP induces hyperphagia and increased gain in body weight in rodents, but may also exert metabolic effects even when hyperphagia is prevented. In the absence of hyperphagia, intracerebralventricular administration of AGRP caused significant increases in plasma leptin and insulin concentrations (two-fold and 1.5-fold, respectively) and fat pad mass. In the perifery, AGRP mRNA was found in adrenal glands, lung, testis, ovary, skeletal muscle and adipose tissue in humans or rodents. In the adrenals, it was shown that AGRP antagonizes glucosteroid production mediated by MC4R. AGRP could then modulate locally the functions of some peripheral tissues such as adrenals. In human and rat serum, detectable levels of AGRP-like activity were reported in the lower picogram range. The serum AGRP levels were elevated in obese humans compared to lean controls and increased with fasting in rats. |
| Immunogen | Swiss Num.: O00253 |
| Source | E. coli |
| Format | State: Lyophilized purified protein Purity: >95% BufferSystem: 5mM TRIS, 25mM NaCl, pH 7.5 Reconstitution: Restore with deionized water |
| Applications | ELISA.
Western Blot. |
| Description | Total 128 AA. MW: 14.4 kDa (calculated). N-Terminal His-tag, 16 extra AA (highlighted). AASequence: MKHHHHHHHM LVPRGSAQMG LAPMEGIRRP DQALLPELPG LGLRAPLKKT TAEQAEEDLL QEAQALAEVL DLQDREPRSS RRCVRLHESC LGQQVPCCDP CATCYCRFFN AFCYCRKLGT AMNPCSRT |
| Storage | Store lyophilized (preferably in a desiccator) at -20°C and in aliquots at -80°C.
Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after two weeks at 4°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Dinulescu DM, Cone RD. Agouti and agouti-related protein: analogies and contrasts.
2. Olszewski PK, Wickwire K, Wirth MM, Levine AS, Giraudo SQ. Agouti-related protein: appetite or reward. 3. Korner J, Wissig S, Kim A, Conwell IM, Wardlaw SL. Effects of agouti-related protein on metabolism and hypothalamic neuropeptide gene expression. 4. Shen CP, Wu KK, Shearman LP, Camacho R, Tota MR, Fong TM, Van der Ploeg LH. Plasma agouti-related protein level: a possible correlation with fasted and fed states in humans and rats. 5. Argyropoulos G, Rankinen T, Bai F, Rice T, Province MA, Leon AS, Skinner JS, Wilmore JH, Rao DC, Bouchard C. The agouti-related protein and body fatness in humans. |
| Pictures | 12% SDS-PAGE separation of Human A GRP
1. M.W. marker - 14, 21, 31, 45, 66, 97 kDa 2. reduced and heated sample, 5µg/lane 3. non-reduced and non-heated sample, 5µg/lane |
