PA521X Secreted Phospholipase A2-IIA (sPLA2-IIA) (His-tagged Fusion Protein) antigen
0.1 mg / US$ 565
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Human Secreted Phospholipase A2-IIA (sPLA2-IIA) (His-tagged Fusion Protein)
Product Description
Human Secreted Phospholipase A2-IIA (sPLA2-IIA) (His-tagged Fusion Protein)
Properties
| Presentation | Purified |
| Species | Human |
| Purity | >95% |
| Source | E. coli |
| Catalog Number | PA521X |
| Quantity | 0.1 mg |
| Price | US$ 565 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  PA521X.pdf |
Datasheet Extract
| Alternate names | Secreted Phospholipase A2-IIA |
| Concentration | 0.5 mg/mL |
| Background | Phospholipase A2 (PLA2) catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to liberate arachidonic acid (AA), a precursor of eicosanoids including prostaglandins and leukotrienes. The same reaction also produces lysophosholipids, which represent another class of lipid mediators. The secretory PLA2 (sPLA2) family, in which 10 isozymes have been identified, consists of low-molecular weight, Ca2+-requiring secretory enzymes that have been implicated in a number of biological processes, such as modification of eicosanoid generation, inflammation, and host defense. This enzyme has been proposed to hydrolyze phosphatidylcholine (PC) in lipoproteins to liberate lyso-PC and free fatty acids in the arterial wall, thereby facilitating the accumulation of bioactive lipids and modified lipoproteins in atherosclerotic foci. In mice, sPLA2 expression significantly influences HDL particle size and composition and demonstrate that an induction of sPLA2 is required for the decrease in plasma HDL cholesterol in response to inflammatory stimuli. Instillation of bacteria into the bronchi was associated with surfactant degradation and a decrease in large:small ratio of surfactant aggregates in rats. |
| Source | E. coli |
| Format | State: Lyophilized purified protein Purity: >95% BufferSystem: 0.05M Acetate buffer pH4 |
| Applications | Western Blot. |
| Description | Total 140 AA. MW: 15.8 kDa (calculated). N-Terminal His-tag, 16 extra AA (highlighted). AASequence: MRGSHHHHHH GMASHMNLVN FHRMIKLTTG KEAALSYGFY GCHCGVGGRG SPKDATDRCC VTHDCCYKRL EKRGCGTKFL SYKFSNSGSR ITCAKQDSCR SQLCECDKAA ATCFARNKTT YNKKYQYYSN KHCRGSTPRC |
| Add. Information | Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell cul |
| Storage | Store lyophilized (preferably in a desiccator) at -20°C and in aliquots at -80°C.
Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after two weeks at 4°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Ronkko S, Rekonen P, Kaarniranta K, Puustjarvi T, Terasvirta M, Uusitalo H . Phospholipase A2 in chamber angle of normal eyes and patients with primary open angle glaucoma and exfoliation glaucoma. Mol Vis . Mar 26;13:408-17 (2007)
2. Koduri RS, Gronroos JO, Laine VJ, Le Calvez C, Lambeau G, Nevalainen TJ, Gelb MH. Bactericidal properties of human and murine groups I, II, V, X, and XII secreted phospholipases A(2). J Biol Chem. 2002 Feb 22;277(8):5849-57. 3. Murakami M, Masuda S, Shimbara S, Bezzine S, Lazdunski M, Lambeau G, Gelb MH, Matsukura S, Kokubu F, Adachi M, Kudo I. Cellular arachidonate-releasing function of novel classes of secretory phospholipase A2s (groups III and XII). J Biol Chem. 2003 Mar; 278(12): 10657-67. 4. Ishizaki J, Suzuki N, Higashino K, Yokota Y, Ono T, Kawamoto K, Fujii N, Arita H, Hanasaki K. Cloning and characterization of novel mouse and human secretory phospholipase A(2)s. J Biol Chem. 1999 Aug; 274(35):24973-9. 5. Ito M, Ishikawa Y, Kiguchi H, Komiyama K, Murakami M, Kudo I, Akasaka Y, Ishii T. Distribution of type V secretory phospholipase A2 expression in human hepatocytes damaged by liver disease. J Gastroenterol Hepatol. 2004 Oct;19(10):1140-9. 6. Yagami T, Ueda K, Asakura K, Hayasaki-Kajiwara Y, Nakazato H, Sakaeda T, Hata S, Kuroda T, Takasu N, Hori Y. Group IB secretory phospholipase A2 induces neuronal cell death via apoptosis. J Neurochem. 2002 May; 81(3): 449-61. 7. Wooton-Kee CR, Boyanovsky BB, Nasser MS, de Villiers WJ, Webb NR. Group V sPLA2 hydrolysis of low-density lipoprotein results in spontaneous particle aggregation and promotes macrophage foam cell formation. Arterioscler Thromb Vasc Biol. 2004 Apr;24(4):762-7. 8. Yagami T, Ueda K, Asakura K, Hata S, Kuroda T, Sakaeda T, Takasu N, Tanaka K, Gemba T, Hori Y. Human group IIA secretory phospholipase A2 induces neuronal cell death via apoptosis. Mol Pharmacol. 2002 Jan; 61(1): 114-26. 9. Lindbom J, Ljungman AG, Lindahl M, Tagesson C. Increased gene expression of novel cytosolic and secretory phospholipase A(2) types in human airway epithelial cells induced by tumor necrosis factor-alpha and IFN-gamma. J Interferon Cytokine Res. 2002 Sep; 22(9): 947-55. 10. Attalah HL, Wu Y, Alaoui-El-Azher M, Thouron F, Koumanov K, Wolf C, Brochard L, Harf A, Delclaux C, Touqui L. Induction of type-IIA secretory phospholipase A2 in animal models of acute lung injury. Eur Respir J. 2003 Jun; 21(6): 1040-5. 11. Balboa MA, Shirai Y, Gaietta G, Ellisman MH, Balsinde J, Dennis EA. Localization of group V phospholipase A2 in caveolin-enriched granules in activated P388D1 macrophage-like cells. J Biol Chem. 2003 Nov 28;278(48):48059-65. 12. Touqui L, Alaoui-El-Azher M. Mammalian secreted phospholipases A2 and their pathophysiological significance in inflammatory diseases. Curr Mol Med. 2001 Dec; 1(6): 739-54. Review. 13. Murakami M, Kudo I. New phospholipase A(2) isozymes with a potential role in atherosclerosis. Curr Opin Lipidol. 2003 Oct; 14(5): 431-6. 14. Valentin E, Ghomashchi F, Gelb MH, Lazdunski M, Lambeau G. On the diversity of secreted phospholipases A(2). Cloning, tissue distribution, and functional expression of two novel mouse group II enzymes. **Cloning, tissue distribution, and functional expression of two novel mouse group II enzymes.J Biol Chem. 1999 Oct; 274(44): 31195-202. 15. Erratum in: J Biol Chem 2000 Jan 275(3): 2246. Hanasaki K, Arita H. Phospholipase A2 receptor: a regulator of biological functions of secretory phospholipase A2. Prostaglandins Other Lipid Mediat. 2002 Aug; 68-69: 71-82. Review. 16. Silliman CC, Moore EE, Zallen G, Gonzalez R, Johnson JL, Elzi DJ, Meng X, Hanasaki K, Ishizaki J, Arita H, Ao L, England KM, Banerjee A. Presence of the M-type sPLA(2) receptor on neutrophils and its role in elastase release and adhesion. Am J Physiol Cell Physiol. 2002 Oct; 283(4): C1102-13. 17. Sawada H, Murakami M, Enomoto A, Shimbara S, Kudo I. Regulation of type V phospholipase A2 expression and function by proinflammatory stimuli. Eur J Biochem. 1999 Aug;263(3):826-35. 18. Satake Y, Diaz BL, Balestrieri B, Lam BK, Kanaoka Y, Grusby MJ, Arm JP. Role of group V phospholipase A2 in zymosan-induced eicosanoid generation and vascular permeability revealed by targeted gene disruption. J Biol Chem. 2004 Apr 16;279(16):16488-94. 19. Kuwata H, Sawada H, Murakami M, Kudo I. Role of type IIA secretory phospholipase A2 in arachidonic acid metabolism. Adv Exp Med Biol. 1999; 469: 183-8. 20. Beck GCh, Yard BA, Schulte J, Haak M, van Ackern K, van der Woude FJ, Kaszkin M. Secreted phospholipases A2 induce the expression of chemokines in microvascular endothelium. Biochem Biophys Res Commun. 2003 Jan 17; 300(3): 731-7. 21. Juhl K, Efanov AM, Olsen HL, Gromada J. Secretory phospholipase A2 is released from pancreatic beta-cells and stimulates insulin secretion via inhibition of ATP-dependent K+ channels. Biochem Biophys Res Commun. 2003 Oct 17; 310(2): 274-9. |
| Pictures | 12% SDS-PAGE separation of Human sPLA2-IIA 1. M.W. marker - 14, 21, 31, 45, 66, 97 kDa 2. reduced and heated sample, 10µg/lane 3. non-reduced and non-heated sample, 10µg/lane |
