PA532 rec. REG-1 alpha His-Tagged Fusion Protein antigen
0.1 mg / US$ 565
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Human rec. REG-1 alpha His-Tagged Fusion Protein
Synonyms
REG1a, REG-1a, REG-1a,
Product Description
Human rec. REG-1 alpha His-Tagged Fusion Protein
Properties
| Presentation | Purified |
| Species | Human |
| Purity | >95% |
| Source | E. coli |
| Molecular Weight | 17.8 kD |
| Catalog Number | PA532 |
| Quantity | 0.1 mg |
| Price | US$ 565 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  PA532.pdf |
Datasheet Extract
| Alternate names | REG 1 alpha, PSP, Lithostatine, PTP |
| Concentration | 0.5 mg/mL |
| Background | Reg protein was shown to be stimulated during the regeneration of pancreatic islets. Since then, many Regrelated proteins have been identified in humans and other animals. In human, the four REG family genes, i.e., REG 1 alpha, REG 1 beta, REG-related sequence (RS) and HIP/PAP, have so far been isolated. These Regrelated proteins are classified into four subfamilies according to their amino-acid sequences, but they share a similar structure and physiological function. Reg protein is a growth factor for pancreatic beta cells and also suggest that the administration of Reg protein could be used as another therapeutic approach for diabetes mellitus. human REG cDNA which encoded a 166-amino acid protein with a 22-amino acid signal peptide. The amino acid sequence of human REG protein has 68% homology to that of rat Reg protein. Reg I was found to be expressed mainly in pancreatic beta and acinoductular cells as well as gastric fundic enterochromaffin-like (ECL) cells. Reg I production in ECL cells is stimulated by gastrin, as well as by the proinflammatory cytokine, cytokine-induced neutrophil chemoattractant (CINC)-2Beta. In patients with chronic hypergastrinemia, Reg production is stimulated, with the increased proliferation of gastric mucosal cells. Patients with Helicobacter pylori infection also showed increased Reg production in the gastric mucosa, partly via increased plasma gastrin concentration and partly via increased proinflammatory cytokine production. The serum concentration of the reg-protein was significantly higher in patients with various pancreatic diseases than in normal controls, and was also significantly higher in patients with acute pancreatitis or chronic relapsing pancreatitis than in patients with chronic pancreatitis. Furthermore, the serum PSP/reg-protein concentration was also significantly increased in liver cirrhosis, choledocholithiasis, and various cancers of the digestive system. |
| Source | E. coli |
| Format | State: Lyophilized purified protein Purity: >95% BufferSystem: 5mM Tris, 25mM NaCl, pH 7.5 Reconstitution: Restore with deionized water |
| Applications | ELISA.
Western Blot. |
| Description | Total 156 AA. MW: 17.8 kDa (calculated). N-Terminal His-tag 12 AA (highlighted). AASequence: MKHHHHHHAS HMQEAQTELP QARISCPEGT NAYRSYCYYF NEDRETWVDA DLYCQNMNSG NLVSVLTQAE GAFVASLIKE SGTDDFNVWI GLHDPKKNRR WHWSSGSLVS YKSWGIGAPS SVNPGYCVSL TSSTGFQKWK DVPCEDKFSF VCKFKN |
| Specificity | The amino acid sequence of the recombinant Human REG 1 alpha is 100% homologous to the amino acid sequence of the Human REG 1 alpha withoutsignal sequence. |
| Add. Information | Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. |
| Storage | Store lyophilized (preferably in a desiccator) at -20°C and in aliquots at -80°C.
Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after two weeks at 4°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Boonyasrisawat W, Pulsawat P, Yenchitsomanus PT, Vannasaeng S, Pramukkul P,Deerochanawong C, Sriussadaporn S, Ploybutr S, Pasurakul T, Banchuin N . Analysis of the reg1alpha and reg1beta gene transcripts in patients with fibrocalculous pancreatopathy. Southeast Asian J Trop Med Public Health. 2002 Jun;33(2):365-72.
2. Zhang YW, Ding LS, Lai MD. Reg gene family and human diseases. World J Gastroenterol. 2003 Dec;9(12):2635-41. 3. Kinoshita Y, Ishihara S, Kadowaki Y, Fukui H, Chiba T. Reg protein is a unique growth factor of gastric mucosal cells. J Gastroenterol. 2004 Jun;39(6):507-13. 4. Okamoto H. The Reg gene family and Reg proteins: with special attention to the regeneration of pancreatic beta-cells. J Hepatobiliary Pancreat Surg. 1999;6(3):254-62. |
| Pictures | 12% SDS-PAGE separation of Human REG1 alpha 1. M.W. marker - 14, 21, 31, 45, 66, 97 kDa 2. reduced and heated sample, 2.5µg/lane 3. non-reduced and non-heated sample, 2.5µg/lane |
