PP1054P2 Macrophage Inflammatory Protein-4 (MIP-4) (PARC) antibody
see related secondary antibodiessee all 7 Macrophage Inflammatory Protein-4 products
0.1 mg / US$ 495
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti Macrophage Inflammatory Protein-4 (MIP-4) (PARC)
Synonyms
MIP4, CCL18,
Product Description
Rabbit anti Macrophage Inflammatory Protein-4 (MIP-4) (PARC) , Presentation: Aff - Purified. Product is tested for Enzyme Immunoassay ( E ), Functional assay ( FN ), Western blot / Immunoblot ( WB )
Properties
| Applications | Enzyme Immunoassay ( E ), Functional assay ( FN ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ) |
| Presentation | Aff - Purified |
| Host | Rabbit |
| Catalog Number | PP1054P2 |
| Quantity | 0.1 mg |
| Price | US$ 495 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  PP1054P2.pdf |
Datasheet Extract
| Alternate names | PARC,
CCl18 |
| Immunogen | Highly pure (>98%) recombinant hMIP-4 (human Macrophage Inflammatory Protein-4). |
| Format | State: Lyophilized purified Ig fraction. Purification: Affinity chromatography. BufferSystem: PBS, pH 7.2 without preservatives. Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Applications | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-4 (100.0 ng/ml), a concentration of 8.0-10.0 µg/ml of this antibody is required.
ELISA: (Indirect): To detect hMIP-4 by indirect ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-4. (Sandwich): To detect hMIP-4 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MIP-4 (PP1054B) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-4. Western Blot: To detect hMIP-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
| Specificity | Specific for human Macrophage Inflammatory Protein-4 (MIP-4) |
| Add. Information | Centrifuge vial prior to opening! |
| Storage | Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer.
Avoid repeated freezing and thawing. Shelf life: One year from despatch. |
| References | 1. Struyf S PARC/CCL18 is a plasma CC chemokine with increased levels in childhood acute lymphoblastic leukemia Am J Pathol, 2003; 163(5):2065-75.
2. Marisa Vulcano Unique regulation of CCL18 production by maturing dendritic cells J. of Immunology, 2003, 170 (7) pp. 3843-3849. 3. Evemie Schutyser Identification of biologically active chemokine isoforms from ascitic fluid and elevated levels of CCL18/pulmonary and activation-regulated chemokine in ovarian carcinoma J. Biol. Chem., 2002, Vol: 277 (27) pp. 24584-24593. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
