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Product Description for Apolipoprotein E (Apo E)
Properties for Apolipoprotein E (Apo E)
| Manufacturer | Acris Antibodies GmbH |
| Quantity | 0.5 mg |
| Reactivity | Human |
| Presentation | Purified |
| Applications | Frozen sections, Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot, Immunoprecipitation |
| Host | Goat |
| PDF datasheet | view  |
| Shipping to | Worldwide |
Datasheet Extract
| Synonyms | ApoE, Apo-E, FocusOn036 |
| Swiss Prot Num | P02649 |
| Immunogen | Apolipoprotein Type E was isolated from human plasma by density gradient centrifugation followed by HPLC purification. |
| Product Type | Antibodies |
| Storage | Store the antibody (undiluted) at 2-8°C for up to one month. For extended storage, mix with an equal volume of glycerol, aliquots contents and freeze at -20°C or below. Avoid repeated freezing and thawing. Dilute only prior to immediate use. Shelf life one year from despatch. |
| Format | State: Liquid (sterile filtered) purified Ig fraction |
| Purification: Immunoaffinity chromatography | |
| Buffer System: 0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA, pH 8.0, containing 0.01% Sodium Azide as preservtive | |
| Specificity | This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other Apolipoproteins and human serum proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human Apolipoprotein E and has negligible cross-reactivity with Type A-1 A-II, B, C-I, C-II, C-III and J Apolipoproteins. Specific cross reaction of anti-Apolipoprotein antibodies with antigens from other species has not been determined. Non-specific cross reaction of anti-Apolipoprotein antibodies with other human serum proteins is negligible. |
| Application | Anti-apoLipoprotein antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis. Recommended dilutions: ELISA: 1/4,000-1/8,000 Western blot: 1/500-1/2,000 Immunohistochemistry: 1/50-1/200 |
| Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
| Focuson | 036 Antibodies to Apolipoprotein |
Secondary Antibodies for Apolipoprotein E (Apo E)
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1317AP | Goat IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / 180 Euro | |||||
| R1317B | Goat IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / 170 Euro | |||||
| R1317F | Goat IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / 160 Euro | |||||
| R1317HRP | Goat IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / 170 Euro | |||||
| R1317T | Goat IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / 160 Euro | |||||
| R1416R | Goat IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / 290 Euro | |||||
| R1435C2 | Goat IgG (H&L) multi-species ads. | ||||||
| Donkey | Cy2 | 1 mg / 250 Euro | |||||
| R1435C3 | Goat IgG (H | ||||||
| Donkey | Cy3 | 1 mg / 250 Euro | |||||
| R1435C5 | Goat IgG (H&L) multi-species ads. | ||||||
| Donkey | Cy5 | 1 mg / 250 Euro | |||||