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Product Description for Collagen type VI
Properties for Collagen type VI
| Manufacturer | Acris Antibodies GmbH |
| Quantity | 0.1 mg |
| Reactivity | Human, Mamalia |
| Presentation | Biotin |
| Applications | Frozen sections, Enzyme Immunoassay, Western blot / Immunoblot |
| Host | Rabbit |
| PDF datasheet | view  |
| Shipping to | Worldwide |
Datasheet Extract
| Synonyms | AB6583 |
| Immunogen | Collagen type VI purified from human and bovine placenta. |
| Product Type | Antibodies |
| Storage | Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer. Dilute only prior to immediate use. Avoid repeated freezing and thawing. Shelf life: One year from despatch. |
| Format | State: Liquid (sterile filtered) purified Ig fraction. |
| Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). | |
| Molar radio: 10-20 BAC molecules per Rabbit IgG molecule . | |
| Purification: Immunoaffinity chromatography. | |
| Buffer System: 0.125M Borate, 0.075M Sodium Chloride, 0.005M EDTA, pH 8.0 containing 10mg/ml BSA as stabilizer and 0.01 % sodium azide as preservative. | |
| Specificity | This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type VI collagens and has negligible cross-reactivity with Type I, II, III, IV and V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible. |
| Application | Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis. Recommended Dilutions: This product was assayed by immunoblot and found to be reactive against Collagen VI at a dilution of 1:5,000 to 1:10,000. This product was also assayed against 1.0 ug of Collagen VI in a standard sandwich ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:12,000 of the stock concentration is suggested for this product. For Immunohistochemistry on frozen tissue sections dilute the product 1:50 to 1:200. |
| Concentration | 1.5 mg/ml (OD280) |
Secondary Antibodies for Collagen type VI
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / 190 Euro | |||||
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / 160 Euro | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / 160 Euro | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / 170 Euro | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / 160 Euro | |||||
| R1427R | Rabbit IgG F(ab)2 Fragment | ||||||
| Donkey | PE | 1 ml / 290 Euro | |||||
| R1458C2 | Rabbit IgG (H&L) | ||||||
| Goat | Cy2 | 1 mg / 250 Euro | |||||
| R1458C3 | Rabbit IgG (H&L) | ||||||
| Goat | Cy3 | 1 mg / 250 Euro | |||||
| R1458C5 | Rabbit IgG (H&L) | ||||||
| Goat | Cy5 | 1 mg / 250 Euro | |||||