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R1043B Collagen type VI antibody

see related secondary antibodies
see all at least 13 Collagen type VI products
0.1 mg / €350.00
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Delivery time: 5-7 workingdays
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Quick Overview

Rabbit anti Human,Mamalia Collagen type VI

Product Description for Collagen type VI

Rabbit anti Human,Mamalia Collagen type VI , Presentation: Biotin. Product is tested for Frozen sections,Enzyme Immunoassay,Western blot / Immunoblot

Properties for Collagen type VI

ManufacturerAcris Antibodies GmbH
Quantity0.1 mg
ReactivityHuman, Mamalia
PresentationBiotin
ApplicationsFrozen sections, Enzyme Immunoassay, Western blot / Immunoblot
HostRabbit
PDF datasheetview
Shipping toWorldwide

Datasheet Extract

SynonymsAB6583
Immunogen Collagen type VI purified from human and bovine placenta.
Main categoryAntibodies
StorageStore the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer. Dilute only prior to immediate use. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
FormatState: Liquid (sterile filtered) purified Ig fraction.
 Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC).
 Molar radio: 10-20 BAC molecules per Rabbit IgG molecule .
 Purification: Immunoaffinity chromatography.
 Buffer System: 0.125M Borate, 0.075M Sodium Chloride, 0.005M EDTA, pH 8.0 containing 10mg/ml BSA as stabilizer and 0.01 % sodium azide as preservative.
SpecificitySpecificity: This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type VI collagens and has negligible cross-reactivity with Type I, II, III, IV and V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
ApplicationAnti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis. Recommended Dilutions: This product was assayed by immunoblot and found to be reactive against Collagen VI at a dilution of 1:5,000 to 1:10,000. This product was also assayed against 1.0 ug of Collagen VI in a standard sandwich ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:12,000 of the stock concentration is suggested for this product. For Immunohistochemistry on frozen tissue sections dilute the product 1:50 to 1:200.
Concentration1.5 mg/ml (OD280)

Secondary Antibodies for Collagen type VI

Catalog No.Name / HostPresentationReactivity 
R1364AP Immunoglobulin G (IgG)
  Goat AP 1 mg / 210 Euro
   
R1364B Immunoglobulin G (IgG)
  Goat Biotin 2 mg / 180 Euro
   
R1364F Immunoglobulin G (IgG)
  Goat FITC 2 mg / 170 Euro
   
R1364HRP Immunoglobulin G (IgG)
  Goat HRP 2 mg / 180 Euro
   
R1364T Immunoglobulin G (IgG)
  Goat TRITC 2 mg / 170 Euro
   
R1427R Immunoglobulin G (IgG) F(ab)2 Fragment
  Donkey PE 1 ml / 340 Euro
   
R1458C2 Immunoglobulin G (IgG)
  Goat Cy2 1 mg / 330 Euro
   
R1458C3 Immunoglobulin G (IgG)
  Goat Cy3 1 mg / 330 Euro
   
R1458C5 Immunoglobulin G (IgG)
  Goat Cy5 1 mg / 330 Euro
   
Click here to see all secondary antibodies for 'R1043B Collagen type VI'