R1099B Malate Dehydrogenase antibody
see related secondary antibodiessee all 9 Malate Dehydrogenase products
10 mg / US$ 465
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Sheep anti Malate Dehydrogenase
Product Description
Sheep anti Malate Dehydrogenase , Presentation: Biotin. Product is tested for Enzyme Immunoassay ( E ), Western blot / Immunoblot ( WB )
Properties
| Applications | Enzyme Immunoassay ( E ), Western blot / Immunoblot ( WB ) |
| Reactivity | Por ( Por ) |
| Presentation | Biotin |
| Host | Sheep |
| Catalog Number | R1099B |
| Quantity | 10 mg |
| Price | US$ 465 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1099B.pdf |
Datasheet Extract
| Concentration | 10.0 mg/ml (by UV absorbance at 280 nm) |
| Immunogen | Malate Dehydrogenase [Pig Heart]. |
| Format | State: Lyophilized purified Ig fraction. Purification: Multi-step process. BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Molarratio: 10-20 BAC molecules per Sheep IgG molecule. Reconstitution: Restore with 1.0 ml of deionized water (or equivalent). |
| Applications | Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:: This product has been assayed against 1.0 ug of Malate Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3- ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:11,000 of the reconstitution concentration is suggested for this product. |
| Specificity | This product is an IgG fraction antibody purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Sheep Serum as well as purified and partially purified Malate Dehydrogenase [Pig Heart]. Cross reactivity against Malate Dehydrogenase from other sources is unknown. |
| Storage | Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing. Shelf life: One year from despatch. |
| References | Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990. (Conjugation)
|
7 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1456B | Sheep IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 295 | |||||
| R1456F | Sheep IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1456T | Sheep IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1456TR | Sheep IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1456HRP | Sheep IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1456AP | Sheep IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 335 | |||||
| R1433R | Sheep IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'R1099B Malate Dehydrogenase'.
