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R1099P  Malate Dehydrogenase antibody

see related secondary antibodies
see all 9 Malate Dehydrogenase products
50 mg / US$ 465
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Sheep anti Malate Dehydrogenase

Product Description

Sheep anti Malate Dehydrogenase , Presentation: Ig Fraction. Product is tested for Enzyme Immunoassay ( E ), Western blot / Immunoblot ( WB )

Properties

ApplicationsEnzyme Immunoassay ( E ), Western blot / Immunoblot ( WB )
ReactivityPor ( Por )
PresentationIg Fraction
HostSheep
Catalog NumberR1099P
Quantity50 mg
PriceUS$ 465
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1099P.pdf

Datasheet Extract

Concentration10.0 mg/ml (by UV absorbance at 280 nm)
ImmunogenMalate Dehydrogenase [Pig Heart].
Format
State: Lyophilized purified Ig fraction.
Purification: Multi-step process.
BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative.
Reconstitution: Restore with 5.0 ml of deionized water (or equivalent).
ApplicationsSuitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity.
Recommended Dilutions: This product has been assayed against 1.0 ug of Malate Dehydrogenase [Pig Heart] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) (R1364HRP) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:10,000 to 1:40,000 of the reconstitution concentration is suggested for this product.
SpecificityThis product is an IgG fraction antibody purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Malate Dehydrogenase [Pig Heart].
Cross reactivity against Malate Dehydrogenase from other tissues and species may occur but have not been specifically determined.
StorageStore vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

7 Secondary Antibodies

Catalog No.Name / HostPresentationReactivity 
R1456B Sheep IgG (H&L)  
  Rabbit Biotin   2 mg / US$ 295
   
R1456F Sheep IgG (H&L)  
  Rabbit FITC   2 mg / US$ 285
   
R1456T Sheep IgG (H&L)  
  Rabbit TRITC   2 mg / US$ 285
   
R1456TR Sheep IgG (H&L)  
  Rabbit Texas Red   2 mg / US$ 295
   
R1456HRP Sheep IgG (H&L)  
  Rabbit HRP   2 mg / US$ 315
   
R1456AP Sheep IgG (H&L)  
  Rabbit AP   1 mg / US$ 335
   
R1433R Sheep IgG (H&L) F(ab')2 Fragment multi-species ads.  
  Donkey PE   1 ml / US$ 455
   

Click here to see all secondary antibodies for 'R1099P Malate Dehydrogenase'.