R1185 COX2 / PTGS2 antibody
see related secondary antibodiessee all 35 COX2 / PTGS2 products
0.1 ml / US$ 465
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti COX2 / PTGS2 -
Synonyms
Prostaglandin-endoperoxide synthase 2, , COX-2, PGHS-2, PHS II, , Prostaglandin G/H synthase 2, Cyclooxygenase-2, Prostaglandin H2 synthase 2, PGH synthase 2
Product review
Please read our product review about COX2 / PTGS2 : Antibody Panel to Prostaglandin-Endoperoxidase Synthase Enymes (Cyclooxygenase, PGHS).
Product Description
Rabbit anti COX2 / PTGS2 -, Presentation: Serum. Product is tested for Enzyme Immunoassay ( E ), Western blot / Immunoblot ( WB )
Properties
| Applications | Enzyme Immunoassay ( E ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ), Mouse ( Ms ), Rat ( Rt ) |
| Presentation | Serum |
| Host | Rabbit |
| Catalog Number | R1185 |
| Swiss Prot. No. | P35354 |
| Quantity | 0.1 ml |
| Price | US$ 465 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1185.pdf |
Datasheet Extract
| Polyclonal Antibody to Human Cox-2 | |
| Concentration | 85 mg/ml (by Refractometry) |
| Background | Cox-2 (Cyclooxygenase-2) is an inducible enzyme that is normally absent from cells, however, in response to growth factors, tumor promoters and some cytokines, it undergoes a rapid and transient expression. COX-2, also known as prostaglandin H synthase, was identified less than a decade ago. Its discovery was followed by a period of discovery and drug development to create a "super aspirin" and led to insights into arthritis, Alzheimer's disease, colorectal cancer and regulation of brain and kidney function in search of better treatments for degenerative and inflammatory diseases. Two different isoforms of the enzyme are known, Cox-1 and Cox-2. |
| Immunogen | GST fusion protein; Epitope maps to the carboxy-terminus of human Cox-2. Swiss Num.: P35354 |
| Format | State: Liquid (sterile filtered) Purification: None BufferSystem: Serum |
| Applications | This antiserum against Human Cox-2 and has been tested for use in immunoblotting and ELISA. The antibody recognizes Cox-2 at 70kDa in Sf9 cell lines transfected with Cox-2 as well as in WISH cells induced with IL-1b. Reactivity in other immunoassays is unknown.
Recommended Dilutions: This product was assayed by immunoblot using HRP Goat-anti-Rabbit IgG and ECL for detection. A working dilution of 1:5,000 to 1:10,000 is suggested for this assay. For ELISA a working dilution of 1:10,000 to 1:50,000 is suggested using Protein A HRP and TMB as a substrate for 30 minutes at room temperature. |
| Specificity | This antiserum is directed against human Cox-2 and is useful in determining its presence in western blotting and ELISA experiments. The antibody does not cross react with Cox-1. Cox-2 is involved in the response of cells to growth factors, tumor promoters, and cytokines that induce its expression. Given its role in synthesizing prostaglandins, Cox-2 is therefore of interest in studying immune response regulation. This antibody can detect Cox-2 in human, mouse and rat. Species: Human, mouse and rat. |
| Storage | Store vial at -20°C prior to opening. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. |
| References | (Specific):
Tai, H. et. al., (2000) Induction of NAD(+)-linked 15-hydroxyprostaglandin dehydrogenase expression by androgens in human prostate cancer cells. Biochem Biophys Res Commun. Sep 16;276(1):77-81. Guan, Z. et al. (1998) Interleukin-1-induced Cyclooxygenase-2 Expression Requires Activation of Both c-Jun NH2-terminal Kinase and p38 MAPK Signal Pathways in Rat Renal Mesangial Cells. J.Biol Chem, 273(44): 28670-28676. (Background): Hittelman, A.B. et al., (1999). Differential regulation of glucocorticoid receptor transcriptional activation via AF-1-associated proteins. EMBO J Oct 1;18(19):5380-8. Golden, B.D. and Abramson, S.B. (1999) Selective COX-2 Inhibitors for Arthritis: from Bench to Bedside. Rheuma21st (Internet Journal). Gotlieb, D. Current Cox Concept. COX 1 and 2: The cyclo-oxygenase systems. Online article. Bathon, J.M. Specific Cox-2 Inhibitors. Johns Hopkins University Online article. Includes a detailed reference list for inhibitors of Cox-2. Markenson, J. (1999) Clinical Implications of Cyclooxygenase Enzymes: COX-1/COX-2 Role of the New NSAIDs. Cancer Control: Journal of the Moffitt Cancer Center, March/April 1999 Supplement. Gierse, J. K. et al. (1996) A Single Amino Acid Difference Between Cyclooxygenase-1 (COX-1) and -2 (COX-2) Reverses the Selectivity of COX-2 Specific Inhibitors. J. Biol. Chem. 271:15810-15814. |
| Pictures | Figure- Anti-Cox2 is shown
to detect Cox-2 present in Cox-2 transfected Sf9 cell extract (lane 1) and IL-1β induced WISH cell extract (lane 2). Detection occurs using a 1:10,000 dilution of antibody followed by 1:10,000 dilution of HRP Goat-anti-Rabbit with visualization via ECL. Film exposure approximately 1 min. Other detection systems will yield similar results. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'R1185 COX2 / PTGS2 '.