R1254AP Goat IgG (H&L) multi-species ads. secondary antibody
see all 12 Goat IgG (H&L) multi-species ads. products
1 mg / please contact our distributor
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Donkey anti Goat IgG (H&L) multi-species ads. -
Product Description
Donkey anti Goat IgG (H&L) multi-species ads. -
Properties
| Presentation | AP |
| Host | Donkey |
| Catalog Number | R1254AP |
| Quantity | 1 mg |
| Price | please contact our distributor |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1254AP.pdf |
Datasheet Extract
| Polyclonal Antibody to Goat IgG [H&L] -AP- | |
| Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
| Immunogen | Goat IgG whole molecule. |
| Format | State: Liquid (sterile filtered) purified Ig fraction. Purification: Immunoaffinity chromatography. BufferSystem: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) sodium azide as preservative. Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons). |
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended dilutions: ELISA: 1/3,000-1/12,000. Western Blot: 1/500-1/2,000. Immunohistochemistry: 1/200-1/1,000. |
| Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Donkey Serum, Goat IgG and Goat Serum. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit and Rat Serum Proteins. |
| Storage | Store the antibody undiluted at 2-8°C.
DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Dilute only prior to immediate use. Shelf life: one year from despatch. |
| References | Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971. (Conjugation) |
