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R1254F  Goat IgG (H&L) multi-species ads. secondary antibody

1 mg / US$ 345
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Donkey anti Goat IgG (H&L) multi-species ads. -

Product Description

Donkey anti Goat IgG (H&L) multi-species ads. -, Presentation: FITC

Properties

PresentationFITC
HostDonkey
Catalog NumberR1254F
Quantity1 mg
PriceUS$ 345
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1254F.pdf

Datasheet Extract

Polyclonal Antibody to Goat IgG [H&L] -FITC-
Concentration1.0 mg/ml (by UV absorbance at 280 nm)
ImmunogenGoat IgG whole molecule.
Format
State: Lyophilized purified Ig fraction.
Purification: Immunoaffinity chromatography.
BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
Label: Fluorescein isothiocyanate (FITC, Molecular Weight 390 daltons).
Absorptionemission: 495 nm / 528 nm
Molarratio: 3.9 moles FITC per mole of Donkey IgG.
Reconstitution: Restore with 1.0 ml of deionized water (or equivalent).
ApplicationsSuitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
SpecificityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Donkey Serum, Goat IgG and Goat Serum.
No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit and Rat Serum Proteins.
StorageStore vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
ReferencesThe and Feltkamp, Immunology 18; 865, 1970. (Conjugation)