R1255HRP Rabbit IgG (H&L) secondary antibody
see all 120 Rabbit IgG products
2 mg / US$ 305
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Sheep anti Rabbit IgG (H&L) -
Product Description
Sheep anti Rabbit IgG (H&L) -, Presentation: HRP
Properties
| Presentation | HRP |
| Host | Sheep |
| Catalog Number | R1255HRP |
| Quantity | 2 mg |
| Price | US$ 305 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1255HRP.pdf |
Datasheet Extract
| Polyclonal Antibody to Rabbit IgG [H&L] - HRP | |
| Concentration | 2.0 mg/ml (by UV absorbance at 280 nm) |
| Immunogen | Rabbit IgG whole molecule
|
| Format | State: Lyophilized Ig fraction Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose. Purity: and BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Gentamicin Sulfate as preservative and 10 mg/ml Bovine Serum Albumin (BSA) as stabilizer, IgG and Protease free. Label: Horseradish Peroxidase (HRP) Reconstitution: Restore with 1.0 ml of deionized water (or equivalent). |
| Applications | Suitable for immunoblotting (western or dot blot), ELISA, immuno-peroxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:6,000 to 1:28,000 of the reconstitution concentration is suggested for this product. |
| Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum, Rabbit IgG and Rabbit Serum. |
| Storage | Store vial at 4°C prior to restoration. Restore with deioinzed water (see 'Format'). Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing.
Shelf life: one year from despatch. |
| References | Farr & Nakane, J. Immunol. methods 47; 129-144. 1981. (Conjugation) |
