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R1255TR  Rabbit IgG (H&L) secondary antibody


see all 120 Rabbit IgG products
2 mg / US$ 295
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Sheep anti Rabbit IgG (H&L) -

Product Description

Sheep anti Rabbit IgG (H&L) -, Presentation: Texas Red

Properties

PresentationTexas Red
HostSheep
Catalog NumberR1255TR
Quantity2 mg
PriceUS$ 295
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1255TR.pdf

Datasheet Extract

Polyclonal Antibody to Rabbit IgG [H&L] //-Texas Red(TM)
Concentration1.0 mg/ml (by UV absorbance at 280 nm)
ImmunogenRabbit IgG whole molecule
Format
State: Lyophilized purified IgG fraction
Purification: immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free, and 0.01% (w/v) Sodium Azide
Label: Texas Red(TM) Sulfonyl Chloride (Molecular Weight 625 daltons)
Absorptionemission: 596 nm / 620 nm
Molarratio: 2.2 moles Texas Red per mole of Sheep IgG
Reconstitution: Restore with 2.0 ml of deionized water or equivalent.
ApplicationsSuitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended dilutions:
Flow cytometry 1:2,000 - 1:10,000
IF microscopy 1:500 - 1:2,500
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum, Rabbit IgG and Rabbit Serum.
StorageStore vial at 2-8°C prior to restoration. Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature.
This product is stable for four weeks at 2-8°C as an undiluted liquid.
For extended storage add glycerol to 50% and then aliquot contents and freeze at
-20°C or below. Avoid repeated freezing and thawing.
Dilute only prior to immediate use.
Shelf life: one year from despatch.
ReferencesJ.A. Titus, P.P. Haugland, S.D. Sharrow, D.M. Segal J. Immunol. Methods 50; 193, 1982. (Conjugation)







*Texas Red(TM) is a registered trademark of Molecular Probes, Inc.