R1301AP Chicken IgG (Fc) secondary antibody
see all 52 Chicken IgG products
1 mg / US$ 375
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti Chicken IgG (Fc)
Synonyms
IgY,
Product Description
Rabbit anti Chicken IgG (Fc)
Properties
| Presentation | AP |
| Host | Rabbit |
| Catalog Number | R1301AP |
| Quantity | 1 mg |
| Price | US$ 375 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1301AP.pdf |
Datasheet Extract
| Concentration | 0.4 mg/ml (by UV absorbance at 280 nm) |
| Immunogen | Chicken IgG F(c) fragment. |
| Format | State: Liquid (sterile filtered) purified Ig fraction. Purification: Immunoaffinity chromatography. Purity: / BufferSystem: 0.05M Tris Chloride, 0.15M Sodium Chloride, 1mM Magnesium Chloride, 0.1mM Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, containing 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) and 0.01% (w/v) and 0.01% Sodium Azide as preservative. Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons). |
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Chicken IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:9,000 of the reconstitution concentration is suggested for this product. |
| Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Chicken IgG, Chicken IgG F(c) and Chicken Serum. No reaction was observed against Chicken IgG F(ab')2. |
| Storage | Store the antibody undiluted at 2-8°C.
DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Dilute only prior to immediate use. Shelf life: one year from despatch. |
| References | Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971. (Conjugation)
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