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R1308AP  Feline IgG F(c) secondary antibody

1 mg / US$ 375
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Rabbit anti Feline IgG F(c) -

Product Description

Rabbit anti Feline IgG F(c) -

Properties

PresentationAP
HostRabbit
Catalog NumberR1308AP
Quantity1 mg
PriceUS$ 375
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1308AP.pdf

Datasheet Extract

Polyclonal Antibody to Cat IgG F(c) -AP-
Concentration0.5 mg/ml (by UV absorbance at 280 nm)
ImmunogenCat IgG F(c) fragment.
Format
State: Liquid (sterile filtered) purified Ig fraction.
Purification: Immunoaffinity chromatography.
BufferSystem: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) sodium azide as preservative.
Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons).
ApplicationsSuitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Cat IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,600 to 1:16,000 of the reconstitution concentration is suggested for this product.
SpecificityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Cat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Cat IgG, Cat IgG F(c) and Cat Serum.
No reaction was observed against Cat IgG F(ab')2.
StorageStore the antibody undiluted at 2-8°C.
DO NOT FREEZE!
Freezing alkaline phosphatase conjugates will result in a substantial loss
of enzymatic activity.
Dilute only prior to immediate use.
Shelf life: one year from despatch.
ReferencesModified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971. (Conjugation)