R1310HRP Canine IgG (H&L) secondary antibody
see all 28 Canine IgG products
2 mg / US$ 315
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti Canine IgG (H&L)
Product Description
Rabbit anti Canine IgG (H&L) , Presentation: HRP
Properties
| Presentation | HRP |
| Host | Rabbit |
| Catalog Number | R1310HRP |
| Quantity | 2 mg |
| Price | US$ 315 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1310HRP.pdf |
Datasheet Extract
| Concentration | 2.0 mg/ml (by UV absorbance at 280 nm) |
| Immunogen | Dog IgG whole molecule |
| Format | State: Lyophilized Ig fraction Purification: Immunoaffinity chromatography using Dog IgG coupled to agarose beads BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA (IgG and Protease free) as a stabilizer and 0.01% (w/v) Gentamicin Sulfate as a preservative Label: Horseradish Peroxidase (HRP) Reconstitution: Restore with 1.0 ml of deionized water or equivalent. |
| Applications | Immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. This antibody has been assayed against 1.0 ug of Dog IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:16,000 to 1:72,000 of the reconstitution concentration is suggested for this product. |
| Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Dog IgG and Dog Serum. |
| Storage | Store vial at 4°C prior to restoration. Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for four weeks at 4°C as an undiluted liquid.
For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use. Shelf life: one year from despatch. |
| References | Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981. (Conjugation) |
