R1311HRP Canine IgG F(c) secondary antibody
1.5 mg / US$ 365
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti Canine IgG F(c)
Product Description
Rabbit anti Canine IgG F(c) , Presentation: HRP. Product is tested for Frozen sections ( C ), Enzyme Immunoassay ( E ), Western blot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Enzyme Immunoassay ( E ), Western blot / Immunoblot ( WB ) |
| Presentation | HRP |
| Host | Rabbit |
| Catalog Number | R1311HRP |
| Quantity | 1.5 mg |
| Price | US$ 365 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1311HRP.pdf |
Datasheet Extract
| Concentration | 1.5 mg/ml (by UV absorbance at 280 nm) |
| Immunogen | Dog IgG F(c) fragment |
| Format | State: Lyophilized purified Ig fraction Purification: Immunoaffinity chromatography BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % Gentamicin Sulfate as preservative and 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer Label: Horseradish Peroxidase (HRP) Reconstitution: Restore with 1.0 ml of deionized water (or equivalent). |
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Dog IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:7,000 to 1:40,000 of the reconstitution concentration is suggested for this product. |
| Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Dog IgG coupled to agarose beads.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Dog IgG, Dog IgG F(c) and Dog Serum. No reaction was observed against Dog IgG F(ab')2. |
| Storage | Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing. Shelf life: One year from despatch. |
| Caution | Do Not Use Sodium Azide as Preservative. |
| References | Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981. (Conjugation) |
