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R1312AP  Canine IgG F(ab')2 secondary antibody

1 mg / US$ 355
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Rabbit anti Canine IgG F(ab')2

Product Description

Rabbit anti Canine IgG F(ab')2

Properties

PresentationAP
HostRabbit
Catalog NumberR1312AP
Quantity1 mg
PriceUS$ 355
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1312AP.pdf

Datasheet Extract

Concentration0.5 mg/ml (by UV absorbance at 280 nm)
ImmunogenDog IgG F(ab')2 fragment.
Format
State: Liquid (sterile filtered) purified Ig fraction.
Purification: Immunoaffinity chromatography.
BufferSystem: Format: This antibody is an IgG fraction and was lyophilised from 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) sodium azide as preservative.
Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons).
ApplicationsSuitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/2,000-1/10,000.
Western Blot: 1/500-1/2,500.
Immunohistochemistry: 1/200-1/1,000.
SpecificityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Dog IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Dog IgG, Dog IgG F(ab')2 and Dog Serum.
No reaction was observed against Dog IgG F(c).
StorageStore the antibody undiluted at 2-8°C.
DO NOT FREEZE!
Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Dilute only prior to immediate use.
Shelf life: one year from despatch.
ReferencesModified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971. (Conjugation)