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R1312F  Canine IgG F(ab')2 secondary antibody

2 mg / US$ 315
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Rabbit anti Canine IgG F(ab')2

Product Description

Rabbit anti Canine IgG F(ab')2 , Presentation: FITC

Properties

PresentationFITC
HostRabbit
Catalog NumberR1312F
Quantity2 mg
PriceUS$ 315
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1312F.pdf

Datasheet Extract

Concentration2.0 mg/ml (by UV absorbance at 280 nm)
ImmunogenDog IgG F(ab')2 fragment.
Format
State: Lyophilized purified Ig fraction.
Purification: Immunoaffinity chromatography.
BufferSystem: 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.6 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) sodium azide as preservative.
Label: Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons).
Absorptionemission: 495 nm / 528 nm
Molarratio: 3.5 moles FITC per mole of Rabbit IgG.
Reconstitution: Restore with 1.0 ml of deionized water (or equivalent).
ApplicationsSuitable for Immunomicroscopy (1/500-1/2,500) and Flow cytometry (1/2,000-1/10,000) or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
SpecificityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Dog IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Dog IgG, Dog IgG F(ab')2 and Dog Serum.
No reaction was observed against Dog IgG F(c).
StorageStore vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
ReferencesThe and Feltkamp, Immunology 18; 865, 1970. (Conjugation)