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R1315B  Ferret IgA alpha chain secondary antibody


see all 7 Ferret IgA alpha chain products
1 mg / US$ 315
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Goat anti Ferret IgA alpha chain -

Product Description

Goat anti Ferret IgA alpha chain -, Presentation: Biotin

Properties

PresentationBiotin
HostGoat
Catalog NumberR1315B
Quantity1 mg
PriceUS$ 315
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1315B.pdf

Datasheet Extract

Polyclonal Antibody to Ferret IgA alpha// (alpha chain specific) -Biotin-
Concentration1.0 mg/ml (by UV absorbance at 280 nm)
ImmunogenFerret IgA α chain.
Format
State: Lyophilized purified Ig fraction.
Purification: Immunoaffinity chromatography.
BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) sodium azide as preservative.
Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC).
Molarratio: 10-20 BAC molecules per Goat IgG molecule.
Reconstitution: Restore with 1.0 ml of deionized water (or equivalent).
ApplicationsSuitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Ferret IgA in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:5,000 to 1:20,000 is suggested for this product.
SpecificityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Ferret IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Ferret IgA and Ferret Serum.
No reaction was observed against Ferret IgM or Ferret IgG.
StorageStore vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
ReferencesBayer & Wilchek Methods in Enzymology 184; 138-160, 1990. (Conjugation)