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R1326B  Hamster IgG (H&L) multi-species ads. secondary antibody

1 mg / US$ 355
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Goat anti Hamster IgG (H&L) multi-species ads. -

Product Description

Goat anti Hamster IgG (H&L) multi-species ads. -, Presentation: Biotin

Properties

PresentationBiotin
HostGoat
Catalog NumberR1326B
Quantity1 mg
PriceUS$ 355
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1326B.pdf

Datasheet Extract

Polyclonal Antibody to Hamster IgG [H&L]// -Biotin
Concentration1.0 mg/ml (by UV absorbance at 280 nm)
ImmunogenHamster IgG whole molecule
Format
State: Lyophilized purified IgG fraction
Purification: Immunoaffinity chromatography using Hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/ml BSA (IgG and Protease free) and 0.01% (w/v) Sodium Azide as preservative
Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Molarratio: 10-20 BAC molecules per Goat IgG molecule
Reconstitution: Restore with 1.0 ml of deionized water or equivalent.
ApplicationsImmunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Western blot: 1:1,000 - 1:5,000.
ELISA: 1:10,000 - 1:50,000.
Immunohistochemistry.
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Hamster IgG and Hamster Serum.
No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins.
StorageStore vial at 2-8°C prior to restoration.
Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid.
For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing.
Dilute only prior to immediate use.
Shelf life: one year from despatch.
ReferencesBayer & Wilchek Methods in Enzymology 184; 138-160, 1990. (Conjugation)