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R1326HRP  Hamster IgG (H&L) multi-species ads. secondary antibody

1 mg / US$ 385
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Goat anti Hamster IgG (H&L) multi-species ads. -

Product Description

Goat anti Hamster IgG (H&L) multi-species ads. -, Presentation: HRP

Properties

PresentationHRP
HostGoat
Catalog NumberR1326HRP
Quantity1 mg
PriceUS$ 385
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1326HRP.pdf

Datasheet Extract

Polyclonal Antibody to //Golden Syrian Hamster IgG [H&L] -HRP
Concentration1.0 mg/ml (by UV absorbance at 280 nm)
ImmunogenGolden Syrian Hamster IgG, whole molecule
Format
State: Lyophilized purified Ig fraction
Purification: Immunoaffinity chromatography.
BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Gentamicin Sulfate as preservative.
Label: Horseradish Peroxidase (HRP)
Reconstitution: Restore with 1.0 ml of deionized water (or equivalent).
ApplicationsSuitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended dilutions:
ELISA (1:15,000 - 1:75,000).
Western blot (1:1,000 - 1:5,000).
SpecificityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Golden Syrian Hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Peroxidase, Golden Syrian Hamster IgG and Golden Syrian Hamster Serum.
No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. Greatly reduced reactivity will occur against Armenian Hamster IgG.
StorageStore vial at 2-8°C prior to restoration.
Restore with deionized water or equivalent; centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid.
For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing.
Dilute only prior to immediate use.
Shelf life: One year from despatch.
CautionDo Not Use Sodium Azide as preservative.
ReferencesFarr & Nakane, J. Immunol. Methods 47; 129-144. 1981. (Conjugation)