R1327B Hamster IgG (H&L) secondary antibody
see all 42 Hamster IgG products
1.5 mg / US$ 315
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti Hamster IgG (H&L) -
Product Description
Rabbit anti Hamster IgG (H&L) -, Presentation: Biotin
Properties
| Presentation | Biotin |
| Host | Rabbit |
| Catalog Number | R1327B |
| Quantity | 1.5 mg |
| Price | US$ 315 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1327B.pdf |
Datasheet Extract
| Polyclonal Antibody to Hamster IgG [H&L] - Biotin | |
| Concentration | 1.5 mg/ml (OD 280 nm) |
| Immunogen | Hamster IgG whole molecule
|
| Format | State: Lyophilized IgG fraction Purification: Immunoaffinity chromatography using Hamster IgG coupled to agarose BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free, and 0.01% (w/v) Sodium Azide Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Molarratio: 10-20 BAC molecules per Rabbit IgG molecule Reconstitution: Restore with 1.0 ml of deionized water or equivalent. |
| Applications | Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
This product has been assayed against 1.0µg of hamster IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/15,000 to 1/60,000 of the reconstitution concentration is suggested for this product. |
| Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum, Hamster IgG and Hamster Serum. |
| Storage | Store vial at 4°C prior to restoration. Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid.
For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use. Shelf life: one year from despatch. |
| References | Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990. (Conjugation) |
