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R1327HRP  Hamster IgG (H&L) secondary antibody


see all 42 Hamster IgG products
1.5 mg / US$ 325
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Rabbit anti Hamster IgG (H&L) -

Product Description

Rabbit anti Hamster IgG (H&L) -, Presentation: HRP

Properties

PresentationHRP
HostRabbit
Catalog NumberR1327HRP
Quantity1.5 mg
PriceUS$ 325
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1327HRP.pdf

Datasheet Extract

Polyclonal Antibody to Hamster IgG [H&L] -HRP-
Concentration1.5 mg/ml (by UV absorbance at 280 nm, before lyophil.)
ImmunogenGolden Syrian Hamster IgG whole molecule
Format
State: Lyophilized purified Ig fraction.
Purification: Immunoaffinity chromatography.
BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Gentamicin Sulfate as preservative.
Label: Horseradish Peroxidase (HRP).
Reconstitution: Restore with 1.0 ml of deionized water (or equivalent).
ApplicationsSuitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA 1:16,000 - 1:64,000
WESTERN BLOT 1:1,000 - 1:5,000
IMMUNOHISTOCHEMISTRY 1:500 - 1:2,500
SpecificityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Hamster IgG coupled to agarose.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Hamster IgG and Hamster Serum.
StorageStore vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
CautionDo Not Use Sodium Azide as Preservative.
ReferencesFarr & Nakane, J. Immunol. Methods 47; 129-144. 1981. (Conjugation)