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R1334HRP  Human IgG (H&L) ads. to Ms serum proteins secondary antibody

2 mg / US$ 325
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Rabbit anti Human IgG (H&L) ads. to Ms serum proteins

Product Description

Rabbit anti Human IgG (H&L) ads. to Ms serum proteins , Presentation: HRP

Properties

PresentationHRP
HostRabbit
Catalog NumberR1334HRP
Quantity2 mg
PriceUS$ 325
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1334HRP.pdf

Datasheet Extract

Concentration2.0 mg/ml (by UV absorbance at 280 nm)
ImmunogenHuman IgG whole molecule.
Format
State: Lyophilized purified Ig fraction.
Purification: Immunoaffinity chromatography.
Purity: and
BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml Bovine Serum Albumin (BSA IgG and Protease free) and 0.01% (w/v) Gentamicin Sulfate as preservative.
Label: Horseradish Peroxidase (HRP).
Reconstitution: Restore with 1.0 ml of deionized water (or equivalent).
ApplicationsSuitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:9,000 to 1:41,000 is suggested for this product.
SpecificityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti- Peroxidase, anti-Rabbit Serum, Human IgG and Human Serum.
No reaction was observed against Mouse Serum Proteins.
StorageStore vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
CautionDo Not Use Sodium Azide as preservative.
ReferencesFarr & Nakane, J. Immunol. Methods 47; 129-144. 1981. (Conjugation)