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R1335AP  Human IgG (H&L) multi-species ads. secondary antibody

1 mg / US$ 395
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Goat anti Human IgG (H&L) multi-species ads.

Product Description

Goat anti Human IgG (H&L) multi-species ads.

Properties

PresentationAP
HostGoat
Catalog NumberR1335AP
Quantity1 mg
PriceUS$ 395
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1335AP.pdf

Datasheet Extract

Concentration0.8 mg/ml
ImmunogenHuman IgG whole molecule.
Format
State: Liquid (sterile filtered) purified Ig fraction.
Purification: Immunoaffinity chromatography.
Purity: and
BufferSystem: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, containing 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) and 0.01% (w/v) Sodium Azide as preservative.
Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons).
ApplicationsSuitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:4,000 of the reconstitution concentration is suggested for this product.
SpecificityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Human IgG and Human Serum.
No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Mouse, Rabbit, Rat and Sheep Serum Proteins.
StorageStore the antibody at 2-8°C as an undiluted liquid.
DO NOT FREEZE!
Freezing alkaline phosphatase conjugates will result in a substantial loss
of enzymatic activity.
Dilute only prior to immediate use.
Shelf life: One year from despatch.
ReferencesAvarameas and Ternyrock, Immunochemistry 32; 1175 1971. (Conjugation)