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R1345B  Monkey IgM mu chain secondary antibody


see all 7 Monkey IgM mu chain products
1 mg / US$ 345
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Goat anti Monkey IgM mu chain -

Product Description

Goat anti Monkey IgM mu chain -, Presentation: Biotin

Properties

PresentationBiotin
HostGoat
Catalog NumberR1345B
Quantity1 mg
PriceUS$ 345
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1345B.pdf

Datasheet Extract

Polyclonal Antibody to Monkey IgM mu// (mu chain specific) -Biotin-
Concentration1.0 mg/ml (by UV absorbance at 280 nm)
ImmunogenMonkey IgM µ chain.
Format
State: Lyophilized purified Ig fraction.
Purification: Immunoaffinity chromatography.
BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC).
Molarratio: 10-20 BAC molecules per Goat IgG molecule.
Reconstitution: Restore with 1.0 ml of deionized water (or equivalent).
ApplicationsSuitable for Immunoblotting, ELISA, Immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
This product has been assayed against 1.0 ug of Monkey IgM in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:20,000 of the concentration is suggested for this product.
SpecificityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Monkey IgM and Monkey Serum.
No reaction was observed against Monkey IgA or Monkey IgG.
StorageStore vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
ReferencesConjugation Reference: Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990.