R1348AP Mouse IgG (H&L) multi-species ads. secondary antibody
1 mg / US$ 395
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Donkey anti Mouse IgG (H&L) multi-species ads.
Product Description
Donkey anti Mouse IgG (H&L) multi-species ads.
Properties
| Presentation | AP |
| Host | Donkey |
| Catalog Number | R1348AP |
| Quantity | 1 mg |
| Price | US$ 395 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1348AP.pdf |
Datasheet Extract
| Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
| Immunogen | Mouse IgG whole molecule. |
| Format | State: Liquid (sterile filtered) purified Ig fraction. Purification: Immunoaffinity chromatography. BufferSystem: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml Bovine Serum Albumin (BSA) IgG snd protease free) as stabilizer. Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons). |
| Applications | Suitable for Immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA usingp-nitrophenyl phosphate (pNPP) as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,000 of the reconstitution concentration is suggested for this product. |
| Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Donkey Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Chicken, Donkey, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins. |
| Storage | Store the antibody (undiluted) at 2-8°C.
DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Dilute only prior to immediate use. Shelf life: one year from despatch. |
| References | Conjugation Reference: Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971.
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