R1349B Mouse IgA + IgG + IgM (H&L) secondary antibody
see all 7 Mouse IgA + IgG + IgM (H&L) products
2 mg / please contact our distributor
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Goat anti Mouse IgA + IgG + IgM (H&L) -
Product Description
Goat anti Mouse IgA + IgG + IgM (H&L) -, Presentation: Biotin
Properties
| Presentation | Biotin |
| Host | Goat |
| Catalog Number | R1349B |
| Quantity | 2 mg |
| Price | please contact our distributor |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1349B.pdf |
Datasheet Extract
| Polyclonal Antibody to //Mouse IgG, IgA, IgM [H&L] -Biotin- | |
| Concentration | 2.0 mg/ml (by UV absorbance at 280 nm) |
| Immunogen | Mouse IgG, IgA and IgM whole molecules. |
| Format | State: Lyophilized purified Ig fraction. Purification: Immunoaffinity chromatography. BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01% (w/v) Sodium Azide as preservative. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Molarratio: 10-20 BAC molecules per Goat IgG molecule. Reconstitution: Restore with 1.0 ml of deionized water (or equivalent). |
| Applications | Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:8,000 to 1:16,000 of the reconstitution concentration is suggested for this product. |
| Specificity | This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Goat Serum. This ireagent is suitable for the detection of all mouse immunoglobulin subclasses, isotypes and chain combinations. |
| Storage | Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing. Shelf life: One year from despatch. |
| References | Conjugation Reference: Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990.
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