R1363HRP Rabbit IgG (H&L) secondary antibody
see all 120 Rabbit IgG products
1 mg / US$ 345
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Chicken anti Rabbit IgG (H&L) -
Product Description
Chicken anti Rabbit IgG (H&L) -, Presentation: HRP
Properties
| Presentation | HRP |
| Host | Chicken |
| Catalog Number | R1363HRP |
| Quantity | 1 mg |
| Price | US$ 345 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1363HRP.pdf |
Datasheet Extract
| Polyclonal Antibody to Rabbit IgG [H&L] -HRP- | |
| Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
| Immunogen | Rabbit IgG whole molecule. |
| Format | State: Lyophilized purified Ig fraction. Purification: Immunoaffinity chromatography. BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Gentamicin Sulfate as preservative and 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer. Label: Horseradish Peroxidase (HRP). Reconstitution: Restore with 1.0 ml of deionized water (or equivalent). |
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 is suggested for this product. |
| Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Chicken Serum, Rabbit IgG and Rabbit Serum. |
| Storage | Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing. Shelf life: One year from despatch. |
| References | (Conjugation) Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.
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