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R1365AP  Rabbit IgG (H&L) ads. to Hu serum proteins secondary antibody

1 mg / US$ 335
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Goat anti Rabbit IgG (H&L) ads. to Hu serum proteins -

Product Description

Goat anti Rabbit IgG (H&L) ads. to Hu serum proteins -

Properties

PresentationAP
HostGoat
Catalog NumberR1365AP
Quantity1 mg
PriceUS$ 335
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1365AP.pdf

Datasheet Extract

Polyclonal Antibody to Rabbit IgG [H&L] -AP-
Concentration1.0 mg/ml (by UV absorbance at 280 nm)
ImmunogenRabbit IgG whole molecule.
Format
State: Liquid (sterile filtered) purified IgG fraction.
Purification: Immunoaffinity chromatography.
BufferSystem: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml Bovine Serum Albumin (BSA) IgG an
Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons).
ApplicationsSuitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/2,000- 1/8,000.
Western Blot: 1/500-1/2,000.
Immunohistochemistry: 1/200-1/1,000.
SpecificityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Rabbit IgG and Rabbit Serum.
No reaction was observed against Human Serum Proteins.
StorageStore the antibody (undiluted) at 2-8°C.
DO NOT FREEZE!
Freezing alkaline phosphatase conjugates will result in a substantial
loss of enzymatic activity.
Dilute only prior to immediate use.
Shelf life: one year from despatch.
ReferencesModified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971.