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R1365B  Rabbit IgG (H&L) ads. to Hu serum proteins secondary antibody

2 mg / US$ 305
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Goat anti Rabbit IgG (H&L) ads. to Hu serum proteins -

Product Description

Goat anti Rabbit IgG (H&L) ads. to Hu serum proteins -, Presentation: Biotin

Properties

PresentationBiotin
HostGoat
Catalog NumberR1365B
Quantity2 mg
PriceUS$ 305
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1365B.pdf

Datasheet Extract

Polyclonal Antibody to Rabbit IgG [H&L] -Biotin-
ImmunogenRabbit IgG, whole molecule.
Format
State: Lyophilized purified Ig fraction.
Purification: Immunoaffinity chromatography.
BufferSystem: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA (IgG and Protease free) with 0.01% (w/v) Sodium Azide as preservative.
Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC).
Molarratio: 10-20 BAC molecules per Goat IgG molecule.
ApplicationsSuitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other assays using streptavidin/avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:50,000 to 1:500,000 of the reconstitution concentration is suggested.
SpecificityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Rabbit IgG and Rabbit Serum.
No reaction was observed against Human Serum Proteins.
StorageStore vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
ReferencesBayer & Wilchek Methods in Enzymology 184; 138-160, 1990.