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R1365HRP  Rabbit IgG (H&L) ads. to Hu serum proteins secondary antibody

2 mg / US$ 315
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Goat anti Rabbit IgG (H&L) ads. to Hu serum proteins -

Product Description

Goat anti Rabbit IgG (H&L) ads. to Hu serum proteins -, Presentation: HRP

Properties

PresentationHRP
HostGoat
Catalog NumberR1365HRP
Quantity2 mg
PriceUS$ 315
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1365HRP.pdf

Datasheet Extract

Affinity Purified Goat anti-Rabbit IgG [H&L] - HRP
Concentration2.0 mg/ml (by UV absorbance at 280 nm)
ImmunogenRabbit IgG, whole molecule
Format
State: Lyophilized
Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA (IgG and Protease free) and 0.01% (w/v) Gentamicin Sulfate as preservative
Label: Horseradish Peroxidase (HRP).
Reconstitution: Restore with 1.0 ml of deionized water (or equivalent).
ApplicationsSuitable for immunoblotting (Western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

Recommended dilutions:
ELISA 1/10,000-1/150,000
Western blot 1/1,500-1/5,000
Immunohistochemistry 1/750-1/2,500
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Human Serum Proteins.
Add. InformationMinimum Cross Reactivity to Human Serum Proteins.
Do NOT add Sodium Azide!
StorageStore vial at 2-8°C prior to restoration. For extended storage aliquot contents after reconstitution and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one year from despatch.
References(Conjugation) Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981