R1370HRP Rat IgG (H&L) secondary antibody
see all 196 Rat IgG products
1 mg / US$ 345
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Chicken anti Rat IgG (H&L)
Product Description
Chicken anti Rat IgG (H&L) , Presentation: HRP
Properties
| Presentation | HRP |
| Host | Chicken |
| Catalog Number | R1370HRP |
| Quantity | 1 mg |
| Price | US$ 345 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1370HRP.pdf |
Datasheet Extract
| Concentration | 1.0 mg/ml |
| Immunogen | Rat IgG whole molecules. |
| Format | State: Lyophilized purified Ig fraction. Purification: Immunoaffinity chromatography. Purity: and BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml Bovine Serum Albumin (BSA IgG and Protease free) and 0.01% (w/v) Gentamicin Sulfate as preservative. Label: Horseradish Peroxidase (HRP). Reconstitution: Restore with 1.0 ml of deionized water (or equivalent). |
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:12,000 of the reconstitution concentration is suggested for this product. |
| Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Chicken Serum, Rat IgG and Rat Serum. |
| Storage | Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing. Shelf life: One year from despatch. |
| References | 1. Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.
|
