Acris Antibodies Logo

Antibodies and related products for scientific research: primary antibodies: 80.027, secondary ab: 1.977, proteins: 20.631, kits: 265, other products: 2.216

No staining picture available ...

 

Zoom Out
Zoom In

R1371F  Rat IgG (H&L) secondary antibody


see all 196 Rat IgG products
2 mg / US$ 285
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Rabbit anti Rat IgG (H&L)

Product Description

Rabbit anti Rat IgG (H&L) , Presentation: FITC

Properties

PresentationFITC
HostRabbit
Catalog NumberR1371F
Quantity2 mg
PriceUS$ 285
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1371F.pdf

Datasheet Extract

Concentration2.0 mg/ml (by UV absorbance at 280 nm)
ImmunogenRat IgG whole molecule
Format
State: Lyophilized IgG fraction
Purification: Immunoaffinity chromatography
BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA (IgG and Protease free) and 0.01% (w/v) Sodium Azide
Label: Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons)
Absorptionemission: 495 nm / 528 nm
Molarratio: 3.3 moles FITC per mole of Rabbit IgG
Reconstitution: Restore with 1.0 ml of deionized water or equivalent.
ApplicationsSuitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended dilution(s):
Flow cytometry 1:2,000 - 1:10,000.
Immunofluorescence: 1:500 - 1:2,500.
SpecificityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Rat IgG and Rat Serum.
StorageStore vial at 2-8°C prior to restoration. Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid.
For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing.
Dilute only prior to immediate use.
Shelf life: one year from despatch.
References(Conjugation) The and Feltkamp, Immunology 18; 865, 1970.