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R1371HRP  Rat IgG (H&L) secondary antibody


see all 196 Rat IgG products
2 mg / US$ 315
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Rabbit anti Rat IgG (H&L)

Product Description

Rabbit anti Rat IgG (H&L) , Presentation: HRP. Product is tested for Frozen sections ( C ), Enzyme Immunoassay ( E ), Western blot / Immunoblot ( WB )

Properties

ApplicationsFrozen sections ( C ), Enzyme Immunoassay ( E ), Western blot / Immunoblot ( WB )
PresentationHRP
HostRabbit
Catalog NumberR1371HRP
Quantity2 mg
PriceUS$ 315
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1371HRP.pdf

Datasheet Extract

Concentration2.0 mg/ml (by UV absorbance at 280 nm)
ImmunogenRat IgG whole molecule
Format
State: Lyophilized purified Ig fraction
Purification: Immunoaffinity chromatography
BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 0.01% Gentamicin Sulfate as preservative and 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer.
Label: Horseradish Peroxidase (HRP)
Reconstitution: Restore with 1.0 ml of deionized water (or equivalent).
ApplicationsSuitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidas antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended dilutions:
ELISA: 1:15,000-1:65,000.
Western blot: 1:1,000-1: 5,000.
Immunohistochemistry: 1:500-1:2,500.
SpecificityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Rat IgG and Rat Serum.
StorageStore vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
References1. Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.