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R1375AP  Rat IgM mu chain secondary antibody


see all 8 Rat IgM mu chain products
1 mg / US$ 405
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Rabbit anti Rat IgM mu chain

Product Description

Rabbit anti Rat IgM mu chain

Properties

PresentationAP
HostRabbit
Catalog NumberR1375AP
Quantity1 mg
PriceUS$ 405
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1375AP.pdf

Datasheet Extract

Concentration1.0 mg/ml (by UV absorbance at 280 nm)
ImmunogenRat IgM µ chain.
Format
State: Liquid (sterile filtered) purified Ig fraction.
Purification: Immunoaffinity chromatography.
BufferSystem: .05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free and 0.05% (w/v) Sodium Azide as preservative.
Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons).
ApplicationsSuitable for Immunoblotting (Western or dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
This product has been assayed against 1.0 µg of Rat IgM in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product.
SpecificityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Rat IgM, and Rat Serum.
No reaction was observed against Rat IgG F(c).
StorageStore the antibody (undiluted) at 2-8°C.
DO NOT FREEZE!
Freezing alkaline phosphatase conjugates will result in a substantial
loss of enzymatic activity.
Dilute only prior to immediate use.
Shelf life: one year from despatch.
References1. Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971.