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R1375F  Rat IgM mu chain secondary antibody


see all 8 Rat IgM mu chain products
1.5 mg / US$ 315
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Rabbit anti Rat IgM mu chain

Product Description

Rabbit anti Rat IgM mu chain , Presentation: FITC

Properties

PresentationFITC
HostRabbit
Catalog NumberR1375F
Quantity1.5 mg
PriceUS$ 315
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1375F.pdf

Datasheet Extract

Concentration1.5 mg/ml (by UV absorbance at 280 nm)
ImmunogenRat IgM µ mu chain.
Format
State: Lyophilized purified Ig fraction.
Purification: Immunoaffinity chromatography.
BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free and 0.01% (w/v) Sodium Azide as preservative.
Label: Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons).
Absorptionemission: 495 nm / 528 nm
Molarratio: 3.5 moles FITC per mole of Chicken IgG.
Reconstitution: Restore with 1.0 ml of deionized water (or equivalent).
ApplicationsSuitable for Immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
SpecificityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Rat IgM and Rat Serum.
No reaction was observed against Rat IgG or Rat IgA.
Specificity was confirmed by ELISA at less than 1% cross reactivity against other Rat heavy or light chain isotypes.
StorageStore vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
References1. The and Feltkamp, Immunology 18; 865, 1970.