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R1376AP  Rat IgA alpha chain secondary antibody


see all 7 Rat IgA alpha chain products
1 mg / US$ 385
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Goat anti Rat IgA alpha chain

Product Description

Goat anti Rat IgA alpha chain

Properties

PresentationAP
HostGoat
Catalog NumberR1376AP
Quantity1 mg
PriceUS$ 385
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1376AP.pdf

Datasheet Extract

Concentration1.0 mg/ml (by UV absorbance at 280 nm)
ImmunogenRat IgA alpha chain.




Format
State: Liquid (sterile filtered) purified Ig fraction.
Purification: Immunoaffinity chromatography.
BufferSystem: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (w/v) Glycerol, pH 8.0, containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) sodium azide as preservative.
Label: Alkaline Phosphatase (calf intestine) (Molecular weight 140,000 daltons)
ApplicationsSuitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
This product has been assayed against 1.0 ug of Rat IgA in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature.
A working dilution of 1:2,000 to 1:8,000 is suggested for this product.
SpecificityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Rat IgA and Rat Serum.
Specificity was confirmed by ELISA at less than 1% cross reactivity against other Rat or human heavy or light chain isotypes.
StorageStore the antibody (undiluted) at 2-8°C.
DO NOT FREEZE!
Freezing alkaline phosphatase conjugates will result in a substantial
loss of enzymatic activity.
Dilute only prior to immediate use.
Shelf life: one year from despatch.
References1. Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175, 1971.