R1377B Rat IgA + IgG + IgM (H&L) secondary antibody
see all 7 Rat IgA + IgG + IgM products
2 mg / US$ 355
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Goat anti Rat IgA + IgG + IgM (H&L)
Product Description
Goat anti Rat IgA + IgG + IgM (H&L) , Presentation: Biotin
Properties
| Presentation | Biotin |
| Host | Goat |
| Catalog Number | R1377B |
| Quantity | 2 mg |
| Price | US$ 355 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1377B.pdf |
Datasheet Extract
| Concentration | 2.0 mg/ml (by UV absorbance at 280 nm) |
| Immunogen | Rat IgG, IgA and IgM whole molecules |
| Format | State: Lyophilized purified Ig fraction Purification: Immunoaffinity chromatography BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA IgG and Protease free as stabilizer and 0.01% (w/v) Sodium Azide as preservative Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Molarratio: 10-20 BAC molecules per Goat IgG molecule Reconstitution: Restore with 1.0 ml of deionized water (or equivalent). |
| Applications | Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended dilutions ELISA: 1:150,000. Western blot: 1:2,000 - 1:20,000. Immunohistochemistry: 1:1,000 - 1:10,000. |
| Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Rat IgG, IgA and IgM. This reagent is suitable for the detection of all rat immunoglobulin subclasses, isotypes and chain combinations. |
| Storage | Store vial at 2-8°C prior to restoration.
Restore with deionized water or equivalent; centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing. Dilute only prior to immediate use. Shelf life: One year from despatch. |
| References | 1. Bayer & Wilchek, Methods in Enzymology 184: 138-160 (1990)
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