R1377HRP Rat IgA + IgG + IgM (H&L) secondary antibody
see all 7 Rat IgA + IgG + IgM products
2 mg / US$ 365
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Goat anti Rat IgA + IgG + IgM (H&L)
Product Description
Goat anti Rat IgA + IgG + IgM (H&L) , Presentation: HRP
Properties
| Presentation | HRP |
| Host | Goat |
| Catalog Number | R1377HRP |
| Quantity | 2 mg |
| Price | US$ 365 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1377HRP.pdf |
Datasheet Extract
| Concentration | 2.0 mg/ml (by UV absorbance at 280 nm) |
| Immunogen | Rat IgG, IgA, IgM whole molecules. |
| Format | State: Lyophilized purified Ig fraction. Purification: Immunoaffinity chromatography. BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free), and 0.01% (w/v) Gentamicin Sulfate as a preservative. Label: Horseradish Peroxidase (HRP). Reconstitution: Restore with 1.0 ml of deionized water (or equivalent). |
| Applications | Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:This product has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,500 to 1:6,000 of the reconstitution concentration is suggested for this product. |
| Specificity | This product was prepared from polyspecific antiserum by immunoaffinity chromatography using Rat antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. This reagent is suitable for the detection of all Rat immunoglobulin isotypes and chain combinations. |
| Storage | Store vial at 4°C prior to restoration. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below.
Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 4°C as an undiluted liquid. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. Shelf life: One year from despatch. |
| Caution | Do Not Use Sodium Azide as preservative. |
| References | 1. Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.
|
