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R1377TR  Rat IgA + IgG + IgM (H&L) secondary antibody


see all 7 Rat IgA + IgG + IgM products
2 mg / US$ 355
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Goat anti Rat IgA + IgG + IgM (H&L)

Product Description

Goat anti Rat IgA + IgG + IgM (H&L) , Presentation: Texas Red

Properties

PresentationTexas Red
HostGoat
Catalog NumberR1377TR
Quantity2 mg
PriceUS$ 355
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1377TR.pdf

Datasheet Extract

Concentration2.0 mg/ml (by UV absorbance at 280 nm)
ImmunogenRat IgG, IgA and IgM whole molecules.
Format
State: Lyophilized purified Ig fraction.
Purification: Immunoaffinity chromatography.
BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
Label: Texas Red -- Sulfonyl Chloride (TR; Molecular Weight 625 daltons).
Absorptionemission: 596 nm / 620 nm
Molarratio: 2.9 moles Texas Red? per mole of Goat IgG.
Reconstitution: Restore with 0.5 ml of deionized water (or equivalent).
ApplicationsSuitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1:2,000-1:10,000.
Western Blot: 1:500-1:2,500.
Immunohistochemistry: 1:200-1:1,000.
SpecificityThis product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum.
This product is suitable for the detection of all Rat immunoglobulin classes, isotypes and chain combinations.
StorageStore vial at 4°C prior to restoration. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below.
Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 4°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid cycles of freezing and thawing.
Shelf life: One year from despatch.
References1. J.A. Titus, et al. J. Immunol. Methods 50; 193, 1982.