R1379HRP Sheep IgG (H&L) ads. to Hu serum Proteins secondary antibody
1 mg / US$ 345
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti Sheep IgG (H&L) ads. to Hu serum Proteins
Product Description
Rabbit anti Sheep IgG (H&L) ads. to Hu serum Proteins , Presentation: HRP
Properties
| Presentation | HRP |
| Host | Rabbit |
| Catalog Number | R1379HRP |
| Quantity | 1 mg |
| Price | US$ 345 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1379HRP.pdf |
Datasheet Extract
| Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
| Immunogen | Sheep IgG whole molecules. |
| Format | State: Lyophilized purified Ig fraction. Purification: Immunoaffinity chromatography. Purity: and BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free, 0.01% (w/v) Gentamicin Sulfate as preservative. Label: Horseradish Peroxidase (HRP). Reconstitution: Restore with 1.0 ml of deionized water (or equivalent). |
| Applications | Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Sheep IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:42,000 of the reconstitution concentration is suggested for this product. Western blotting: 1:1,000-1:5,000. Immunohistochemistry: 1:1,1000-1:3,000. |
| Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Sheep IgG and Sheep Serum. No reaction was observed against Human Serum Proteins. |
| Storage | Store vial at 4°C prior to restoration. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below.
Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 4°C as an undiluted liquid. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. Shelf life: One year from despatch. |
| References | 1. Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.
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