R1382F Sheep IgG F(ab')2 secondary antibody
1.5 mg / US$ 315
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti Sheep IgG F(ab')2
Product Description
Rabbit anti Sheep IgG F(ab')2 , Presentation: FITC
Properties
| Presentation | FITC |
| Host | Rabbit |
| Catalog Number | R1382F |
| Quantity | 1.5 mg |
| Price | US$ 315 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1382F.pdf |
Datasheet Extract
| Concentration | 1.5 mg/ml (by UV absorbance at 280 nm) |
| Immunogen | Sheep IgG F(ab')2 fragment. |
| Format | State: Lyophilized purified Ig fraction. Purification: Immunoaffinity chromatography. BufferSystem: 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 with 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01% (w/v) Sodium Azide as preservative. Label: Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons). Absorptionemission: 495 nm / 528 nm Molarratio: 2.8 moles DTAF per mole of Rabbit IgG. Reconstitution: Restore with 1.0 ml of deionized water (or equivalent). |
| Applications | Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. |
| Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Sheep IgG, Sheep IgG F(ab')2 and Sheep Serum. No reaction was observed against Sheep IgG F(c). |
| Storage | Store vial at 4°C prior to restoration. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below.
Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 4°C as an undiluted liquid. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. Shelf life: One year from despatch. |
| References | 1. Blakeslee & Baines, J. Immunol. Methods 13; 305-320, 1976.
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