R1383F Sheep IgG F(ab')2 ads. to Hu serum proteins secondary antibody
0.5 mg / US$ 375
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti Sheep IgG F(ab')2 ads. to Hu serum proteins
Product Description
Rabbit anti Sheep IgG F(ab')2 ads. to Hu serum proteins , Presentation: FITC
Properties
| Presentation | FITC |
| Host | Rabbit |
| Catalog Number | R1383F |
| Quantity | 0.5 mg |
| Price | US$ 375 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1383F.pdf |
Datasheet Extract
| Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
| Immunogen | Sheep IgG F(ab')2 fragment. |
| Format | State: Lyophilized purified Ig fraction. Purification: Immunoaffinity chromatography. BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01% (w/v) Sodium Azide as preservative. Label: Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons). Absorptionemission: 495 nm / 528 nm Molarratio: 3.1 moles FITC per mole of Rabbit IgG. Reconstitution: Restore with 0.5 ml of deionized water (or equivalent). |
| Applications | Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions: Flow Cytometry: 1:2,000-1:10,000. Immunofluorescence microscopy: 1:500-1:2,500. |
| Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Sheep IgG, Sheep IgG F(ab')2 and Sheep Serum. No reaction was observed against Sheep IgG F(c) or Human Serum Proteins. |
| Storage | Store vial at 4°C prior to restoration. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below.
Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 4°C as an undiluted liquid. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. Shelf life: One year from despatch. |
| References | 1. The and Feltkamp, Immunology 18; 865, 1970.
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