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R1384AP  Porcine IgG (H&L) secondary antibody


see all 27 Porcine IgG products
1 mg / US$ 335
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

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Rabbit anti Porcine IgG (H&L) -

Product Description

Rabbit anti Porcine IgG (H&L) -

Properties

PresentationAP
HostRabbit
Catalog NumberR1384AP
Quantity1 mg
PriceUS$ 335
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1384AP.pdf

Datasheet Extract

Polyclonal Antibody to Swine IgG -AP-
Concentration0.65 mg/ml (by UV absorbance at 280 nm)
ImmunogenSwine IgG whole molecule
Format
State: Liquid (sterile filtered) purified Ig fraction.
Purification: Immunoaffinity chromatography.
BufferSystem: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 0.05% (w/v) Sodium Azide as preservative and 10 mg/ml Bovine Serum Albumin (BSA) (IgG and Protease free) as stabilizer.
Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons).
ApplicationsSuitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Swine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:4,000 of the reconstitution concentration is suggested for this product.
SpecificityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Swine IgG, and Swine Serum.
StorageStore the antibody (undiluted) at 2-8°C before opening.
DO NOT FREEZE!
Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Dilute only prior to immediate use.
Shelf life: One year from despatch.
References1. Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971.