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R1384HRP  Porcine IgG (H&L) secondary antibody


see all 27 Porcine IgG products
2 mg / US$ 315
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Rabbit anti Porcine IgG (H&L) -

Product Description

Rabbit anti Porcine IgG (H&L) -, Presentation: HRP

Properties

PresentationHRP
HostRabbit
Catalog NumberR1384HRP
Quantity2 mg
PriceUS$ 315
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1384HRP.pdf

Datasheet Extract

Polyclonal Antibody to Swine IgG -HRP-
Concentration2.0 mg/ml (OD 280 nm)
ImmunogenSwine IgG whole molecule
Format
State: Lyophilized purified Ig fraction
Purification: Immunoaffinity chromatography
BufferSystem: 0.02 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Gentamicin Sulfate as preservative and 10 mg/ml BSA (IgG and Protease free) as stabilizer.
Label: Horseradish Peroxidase (HRP)
Reconstitution: Restore with 1.0 ml of deionized water (or equivalent).
ApplicationsSuitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
ELISA: 1:16,000.
Western blot: 1:1,000 - 1:5,000.
Immunohistochemistry: 1:500 - 1:2,500.
SpecificityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Swine IgG and Swine Serum.
StorageStore vial at 2-8°C prior to restoration. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below.
Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid cycles of freezing and thawing.
Shelf life: One year from despatch.
References1. Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.